CST, 97607T, CaMKI-alpha (F1M8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CAMK1A. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CaMKI-alpha (F1M8F) Rabbit Monoclonal Antibody (CST #97607) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CaMKI-alpha (F1M8F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CaMKI-α protein. This antibody does not cross-react with CaMKI-β, CaMKI-δ, or CaMKI-γ protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CaMKI-α protein. Background The Ca /calmodulin-dependent kinase (CaMK) family, which is activated in response to elevation of intracellular Ca , includes CaMKI, CaMKII, CaMKIV, and CaMK-kinases (CaMKKs) (1,2). CaMKI is a downstream substrate of CaMKK and has four isoforms: CaMKI-α, CaMKI-β, CaMKI-γ, and CaMKI-δ. CaMKI is present in most cell types and may be involved in cellular functions, including transcription, cytoskeletal organization, axonal growth cone motility, and long-term potentiation in neurons (3-6). CaMKII is also ubiquitously expressed in most cell types. While muscular CaMKII has been linked to activation of mitochondrial biogenesis in muscle hypertrophy response, neuronal CaMKII regulates important neuronal functions, including neurotransmitter synthesis, neurotransmitter release, modulation of ion channel activity, cellular transport, cell morphology and neurite extension, synaptic plasticity, learning and memory, and gene expression (7). Like CaMKI, CaMKIV is also a substrate of CaMKKs and is primarily restricted to the nucleus of neurons. CaMKIV regulates gene transcription in neurons through phosphorylation of transcription factors such as CREB and is required for fear memory (8). Alternate Names calcium/calmodulin dependent protein kinase I; calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM kinase I alpha; CaM-KI; CAMK1; CAMK1A; CAMKI; CaMKI-alpha; KCC1A; MGC120317; MGC120318 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG