CST, 9762T, Caspase-6 Antibody

Polyclonal Antibody for studying Casp6. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-6 Antibody (CST #9762) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35 kDa) and the small subunit of caspase-6 resulting from cleavage at aspartic acid 193 (15 kDa). This antibody does not recognize other caspases. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of caspase-6. Antibodies are purified by protein A and peptide affinity chromatography. Background Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane-associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing, and eventual cell death. Alternate Names Apoptotic protease Mch-2; CASP-6; CASP6; caspase 6; caspase 6, apoptosis-related cysteine peptidase; caspase 6, apoptosis-related cysteine protease; Caspase-6; Caspase-6 subunit p11; Caspase-6 subunit p18; MCH2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 15, 35 SOURCE: Rabbit