CST, 97647S, PADI2 (E3P8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PADI2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PADI2 (E3P8Z) Rabbit Monoclonal Antibody (CST #97647) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PADI2 (E3P8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total PADI2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly375 of human PADI2 protein. Background Peptidyl arginine deiminase (PAD) proteins are a family of Ca -dependent enzymes that catalyze the post-translational conversion of arginine to citrulline. There are currently five known PAD isozymes in humans, referred to as PADI1-4 and PADI6 (1). Among these isozymes, peptidyl arginine deiminase type 2 (PADI2) is the most widely expressed, being found in skeletal muscle, brain, colon, breast, macrophages, spleen, and spinal cord tissue, among others (1,2). In normal mouse development, PADI2 expression levels are elevated from 18 days to 2 months of age, and gradually decrease from 3 months onward (3). Some of the most well studied PADI2 substrates include vimentin, actin, myelin basic protein (MBP), glial fibrillary acidic protein (GFAP), and histones (4). PADI2-mediated citrullination has been shown to be involved in neurodegeneration and inflammatory response-associated diseases such as multiple sclerosis (MS), Alzheimer's disease (AD), psoriasis, and rheumatoid arthritis (5). Excessive PAD-mediated deimination of MBP is believed to be a major contributor to MS disease progression, while elevated levels of citrullinated GFAP and vimentin proteins have been found in the brains of AD patients (2,4). PADI2 has also been found to play a role in the progression of several types of cancers, including colorectal, breast, and prostate (5-7). Alternate Names KIAA0994; PAD-H19; PAD2; PADI2; PDI2; peptidlyarginine deiminase type II; peptidyl arginine deiminase 2; peptidyl arginine deiminase, type II; Peptidylarginine deiminase II; protein arginine deiminase; Protein-arginine deiminase type II; Protein-arginine deiminase type-2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 76 Source/Isotype: Rabbit IgG