CST, 97681T, Phospho-RASAL2 (Ser89) (F6K4P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RASAL2 (Ser89) phosphate. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-RASAL2 (Ser89) (F6K4P) Rabbit Monoclonal Antibody (CST #97681) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-RASAL2 (Ser89) (F6K4P) Rabbit mAb recognizes endogenous levels of RASAL2 protein only when phosphorylated at Ser89. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of RASAL2 and may cross-react with phospho-DAB2IP (Ser133). This antibody detects an approximately 120 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser89 of human RASAL2 protein. Background Small GTPases act as molecular switches, regulating processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase-activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. RASAL2 was initially identified as a GAP for the small GTPase Ras and later shown to interact with the Rho-GEF ECT2 and regulate Rho activity in human astrocytoma cells (1).Researchers have implicated RASAL2 as a suppressor of migration and metastasis in human cancer (2) and have shown that RASAL2 downregulation promotes epithelial-mesenchymal transition and metastasis in ovarian (3) and lung cancer (4). Conversely, other research studies show that RASAL2 can be oncogenic in triple-negative breast cancer by activating Rac1 signaling (5). Phosphorylation of RASAL2 at Ser89 has been identified in several phosphoproteomics studies, and this modification may be relevant in human breast and lung cancer (6,7). Alternate Names MGC129919; NGAP; Ras GTPase activating protein-like; Ras GTPase-activating protein nGAP; Ras protein activator like 1; RAS protein activator like 2; RAS protein activator-like 2; RASAL2; RASAL2 protein; RASAL2/ACVR1 fusion Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG