CST, 97687T, ATRIP (E2I9A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATRIP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ATRIP (E2I9A) Rabbit Monoclonal Antibody (CST #97687) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ATRIP (E2I9A) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATRIP protein. This antibody is expected to detect all isoforms of ATRIP protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg275 of human ATRIP protein. Background In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5). Alternate Names AGS1; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; ATRIP; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG