CST, 97728T, PBK/TOPK (F2X2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PBK/TOPK. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PBK/TOPK (F2X2Z) Rabbit Monoclonal Antibody (CST #97728) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity PBK/TOPK (F2X2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total PBK/TOPK protein. Non-specific cytoplasmic staining was observed in human and mouse skeletal muscle and in mouse spleen by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PBK/TOPK protein. Background PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-binding domain, which is thought to interact with the human homologue of the Drosophila Discs large (hDlg) tumor suppressor protein (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclin B has been shown to phosphorylate PBK/TOPK , presumably at Thr9 (5). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4). Alternate Names Cancer/testis antigen 84; CT84; epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; Nori-3; PBK; PDZ binding kinase; PDZ-binding kinase; serine/threonine protein kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; TOPK Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG