CST, 97778T, CD68 (E3O7V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD68 mouse. Validated for WB,IHC,IHC,IF,IF,F,F. Available in 3 sizes. Highly specific and rigorously validated in-house, CD68 (E3O7V) Rabbit Monoclonal Antibody (CST #97778) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:600 - 1:2400 Immunohistochemistry (Paraffin): 1:150 - 1:600 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 29176 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD68 (E3O7V) Rabbit mAb recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry. Species Reactivity: Mouse, Rat, Hamster Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113. Background CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4). Alternate Names Cd68; CD68 antigen; gp11; gp110; Lamp4; Macrosialin; Sca; Scard1 Specification REACTIVITY: M R Hm SENSITIVITY: Endogenous MW (kDa): 70-80, 130-140, 200 Source/Isotype: Rabbit IgG