CST, 9777T, Pirin (1E8) Rat Monoclonal Antibody

Monoclonal Antibody for studying Pirin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Pirin (1E8) Rat Monoclonal Antibody (CST #9777) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Pirin (1E8) Rat Monoclonal Antibody detects endogenous levels of Pirin protein. This antibody does not cross-react with other proteins in the cupin superfamily. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with the full-length Pirin protein. Background Pirin is a highly conserved nuclear protein and a member of the cupin superfamily of proteins, all of which contain two conserved β-barrel fold domains (1). Pirin functions as a co-factor for NFI/CTF1 and Bcl-3, implicating it in DNA replication, transcriptional activation and apoptosis (2,3). Both human and bacterial pirins catalyze the di-oxygenation of quercetin, one of a class of widespread naturally occurring flavenoid compounds that have anti-inflammatory and anti-cancer activities (4). Flavenoids exert these beneficial activities by functioning as antioxidants that stabilize cellular free radical molecules and by directly modulating cell signaling pathways involving PI 3-kinase, Akt/PKB, PKC and MAP kinases (5). Quercetin has also been directly implicated in the regulation of NF-κB activity; thus, Pirin may exert its apoptotic functions both by directly regulating Bcl-3/NF-κB activity and by modulating quercetin levels in the cell (6). Alternate Names PIR; Pirin; pirin (iron-binding nuclear protein); Probable quercetin 2,3-dioxygenase PIR; Probable quercetinase Specification REACTIVITY: H M R Hm Mk B SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rat IgG2a