CST, 97780S, ARID4A/RbBP1 Antibody

Polyclonal Antibody for studying RbBP1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ARID4A/RbBP1 Antibody (CST #97780) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ARID4A/RbBP1 Antibody recognizes endogenous levels of total ARID4A/RbBP1 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly813 of human ARID4A/RbBP1 protein. Antibodies are purified by peptide affinity chromatography. Background ARID4A/RbBP1 was originally identified as an RB binding protein, which could repress E2F-mediated transcription through the recruitment of the mSIN3A complex (1-3). Like other AT-rich interacting domain (ARID) containing proteins, ARID4A/RbBP1 functions as a tumor suppressor, and is frequently mutated in colorectal cancer (4-6). ARID4A/RbBP1 and ARID4B/RbBP1L1 are mainly expressed in Sertoli cells in the testes, where they form a complex and serve as coactivators for androgen receptors to regulate spermatogenesis (7). Alternate Names ARI4A; ARID domain-containing protein 4A; ARID4A; AT rich interactive domain 4A (RBP1-like); AT-rich interaction domain 4A; AT-rich interactive domain-containing protein 4A; RBBP-1; RBBP1; RBP-1; RBP1; retinoblastoma binding protein 1; Retinoblastoma-binding protein 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 205 SOURCE: Rabbit