CST, 97835T, SLC25A1 (E7B2B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC25A1. Validated for WB,WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SLC25A1 (E7B2B) Rabbit Monoclonal Antibody (CST #97835) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:300 - 1:1200 Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SLC25A1 (E7B2B) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLC25A1 protein. Species cross-reactivity for IHC-P is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the mitochondrial matrix-facing domains of human SLC25A1 protein. The antigen has been further characterized as corresponding to residues surrounding Arg173 of human SLC25A1 protein. Background Members of the solute carrier 25 (SLC25) family transport molecules over the mitochondrial inner membrane (1,2). Mutations in these carriers often result in rare but severe pathologies (3). SLC25A1 (tricarboxylate transport protein, CIC, or citrate transport protein) transports citrate from the inner mitochondrial matrix to the cytosol in exchange for malate. This function makes it a key source of cytoplasmic acetyl-CoA and downstream lipid biosynthesis (4). Mutations in SLC25A1 result in D-2- and L-2-hydroxyglutaric aciduria, causing lowered levels of citrate and isocitrate in urine, with elevated urinary levels of other TCA-cycle intermediates (3). Cancer cells have elevated mitochondrial membrane potential and require SLC25A1 to maintain and promote tumorigenesis (5,6). SLC25A1 is a transcriptional target of p53, and inhibiting SLC25A1 activity can blunt mutant p53-driven tumor growth (6). Inhibitors of SLC25A1 have been found to slow the growth of pancreatic and non-small cell lung cancers (7,8). Alternate Names Citrate transport protein; citrate transporter), member 1; CMS23; CTP; D2L2AD; SEA; SLC20A3; SLC25A1; solute carrier family 20 (mitochondrial citrate transporter), member 3; solute carrier family 25 (mitochondrial carrier; Solute carrier family 25 member 1; Tricarboxylate carrier protein; Tricarboxylate transport protein, mitochondrial; TXTP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG