CST, 97885S, ZEB2 (E6U7Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZEB2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ZEB2 (E6U7Z) Rabbit Monoclonal Antibody (CST #97885) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ZEB2 (E6U7Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZEB2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro722 of human ZEB2 protein. Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8). Alternate Names FLJ42816; HSPC082; KIAA0569; SIP-1; SIP1; SMAD interacting protein 1; Smad-interacting protein 1; SMADIP1; ZEB2; ZFHX1B; ZFX1B; zinc finger E-box binding homeobox 2; Zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 180, 210 Source/Isotype: Rabbit IgG