CST, 97952S, SAMHD1 (E8V1G) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying SAMHD1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, SAMHD1 (E8V1G) Rabbit Monoclonal Antibody (PE Conjugate) (CST #97952) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SAMHD1 (E8V1G) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total SAMHD1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SAMHD1 protein. Background SAM domain and HD domain-containing protein 1 (SAMHD1) is a negative regulator of the cell-intrinsic innate immune response (1). Research studies have identified mutations in as a cause of Aicardi-Goutieres syndrome, an autoimmune disease characterized by elevated production of interferon-α and symptoms resembling congenital viral infection (1). SAMHD1 was identified as the restriction factor that renders most myeloid cells refractory to human immunodeficiency virus (HIV) infection (2-4). Expression of the viral protein Vpx in refractory cells targets SAMHD1 for ubiquitin-mediated degradation and relieves HIV restriction. SAMHD1 prevents autoimmunity and HIV infection by hydrolyzing intracellular deoxynucleoside triphosphates (dNTPs), thereby limiting inappropriate immune activation by self nucleic acid and inhibiting reverse transcription of the HIV genome (4-6). Full-length SAMHD1 oligomerizes in a dGTP-dependent manner , leading to high molecular weight dimers, trimers, or tetramers (7). Alternate Names CHBL2; DCIP; Dendritic cell-derived IFNG-induced protein; Deoxynucleoside triphosphate triphosphohydrolase SAMHD1; dNTPase; HDDC1; hSAMHD1; Monocyte protein 5; MOP-5; MOP5; SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1; SAM domain and HD domain 1; SAM domain and HD domain-containing protein 1; SAMH1; SAMHD1; SBBI88 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG