CST, 97958S, SDF1/CXCL12 (D8G6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SDF-1. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, SDF1/CXCL12 (D8G6H) Rabbit Monoclonal Antibody (CST #97958) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 89194 . Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity SDF1/CXCL12 (D8G6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SDF1/CXCL12 protein by immunohistochemistry on paraffin-embedded tissue sections. This antibody recognizes both SDF1/CXCL12α and SDF1/CXCL12β. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SDF1/CXCL12 protein. Background The stromal cell derived factor 1 (SDF1/CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1/CXCL12 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1/CXCL12 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1/CXCL12 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1/CXCL12 isoforms (SDF1/CXCL12α and SDF1/CXCL12β) that have different properties and biological activities (7). Additional isoforms of SDF1/CXCL12 have been reported (8,9). Alternate Names C-X-C motif chemokine 12; C-X-C motif chemokine ligand 12; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; SCYB12; SDF-1; SDF-1-alpha(3-67); SDF-1-beta(3-72); SDF-1a; SDF-1b; SDF1; SDF1A; SDF1B; Stromal cell-derived factor 1; stromal cell-derived factor 1 delta; stromal cell-derived factor 1 gamma; stromal cell-derived factor 1a; TLSF; TLSF-a; TLSF-b; TPAR1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG