CST, 98051S, xCT/SLC7A11 Antibody

Polyclonal Antibody for studying SLC7A11 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, xCT/SLC7A11 Antibody (CST #98051) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity xCT/SLC7A11 Antibody recognizes endogenous levels of total mouse xCT/SLC7A11 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of mouse xCT/SLC7A11 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The X cystine/glutamate antiporter consists of a light chain subunit (xCT/SLC7A11) that confers substrate specificity and a glycosylated heavy chain subunit (4F2hc/SLC3A2) located on the cell surface (1,2). The heterodimeric amino acid transport system X provides selective import of cystine into cells in exchange for glutamate and regulates intracellular glutathione (GSH) levels, essential for cellular protection from oxidative stress (3). Research studies have shown that xCT expression increases in various tumors, including gliomas, and have implicated xCT in GSH-mediated anticancer drug resistance (4,5). Researchers have found that xCT provides neuroprotection by enhancing glutathione export from non-neuronal cells (6). Moreover, investigators identified xCT as the fusion-entry receptor for Kaposi's sarcoma-associated herpesvirus (7). Alternate Names 9930009M05Rik; AI451155; amino acid transport system xc cysteine/glutamate transporter; Amino acid transport system xc-; cysteine/glutamate transporter; Cystine/glutamate transporter; OTTMUSP00000023507; Slc7a11; sodium independent anionic amino acid transport system; solute carrier family 7 (cationic amino acid transporter, y+ system), member 11; Solute carrier family 7 member 11; sut; xCT Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 35 SOURCE: Rabbit