CST, 98074SF, Phospho-Glycogen Synthase (Ser641) (D4H1B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Glycogen Synthase (Ser641) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Phospho-Glycogen Synthase (Ser641) (D4H1B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #98074) is ready to ship. Product Usage Information This product is the carrier free version of product #47043. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 47043 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-Glycogen Synthase (Ser641) (D4H1B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of both muscle and liver isoforms of glycogen synthase protein only when phosphorylated at Ser641. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser641 of human liver glycogen synthase. Background Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals: muscle isoform (glycogen synthase 1, GYS1) and liver isoform (glycogen synthase 2, GYS2), respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649, and Ser653), inhibiting its activity (2,3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1). Alternate Names Glycogen [starch] synthase, muscle; glycogen synthase 1; glycogen synthase 1 (muscle); GSY; GYS; GYS1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85-90 Source/Isotype: Rabbit IgG