CST, 98085S, CAPAM/PCIF1 Antibody

Polyclonal Antibody for studying PCIF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CAPAM/PCIF1 Antibody (CST #98085) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CAPAM/PCIF1 Antibody recognizes endogenous levels of total CAPAM/PCIF1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro229 of human CAPAM/PCIF1 protein. Antibodies are purified by peptide affinity chromatography. Background Phosphorylated CTD Interacting Factor 1 (PCIF1), also known as CAPAM, is a WW domain-containing protein that was initially discovered to interact with phosphorylated RNA Polymerase II, where it inhibits CTD phosphatase activity of SCP1 to negatively affect gene expression (1,2). CAPAM/PCIF1 has shown to be the methyltransferase responsible for methylating adenosine at the second position of mRNAs, promoting their translation (3,4). Alternate Names C20orf67; Cap-specific adenosine methyltransferase; CAPAM; hCAPAM; hPCIF1; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; PCIF1; PDX-1 C terminus-interacting factor 1; PDX1 C-terminal inhibiting factor 1; phosphorylated CTD interacting factor 1; Phosphorylated CTD-interacting factor 1; PPP1R121; Protein phosphatase 1 regulatory subunit 121; protein phosphatase 1, regulatory subunit 121 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85 SOURCE: Rabbit