CST, 98134T, Cleaved Caspase-8 (Asp374) (E6H8S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp8 (Asp374) cleaved. Validated for WB,WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp374) (E6H8S) Rabbit Monoclonal Antibody (CST #98134) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62901 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cleaved Caspase-8 (Asp374) (E6H8S) Rabbit Monoclonal Antibody recognizes endogenous levels of caspase-8 protein only when cleaved at Asp374. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43/p41) as well as the p18 subunit alone. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp374 of human caspase-8 protein. Background Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. In addition to functioning as a key initiator caspase for extrinsic apoptosis, more recent studies have identified caspase-8 as a regulator of inflammatory necrotic cell death pathways such as necroptosis and pyroptosis (4,5). Activation of caspase-8 leads to cleavage of RIPK1 and RIPK3 to inhibit necroptosis (6,7). As a result, caspase-8 deficiency in mice, which is embryonic lethal, can be rescued by deletion of the necroptosis proteins RIPK3 or MLKL (8-11). Additionally, in some circumstances, caspase-8 is recruited to the inflammasome to trigger pyroptosis (12,13). Studies have also found that expression of an enzymatically inactive form of caspase-8 (C362S) causes embryonic lethality by inducing necroptosis and pyroptosis (14). Alternate Names ALPS2B; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; CASP8; caspase 8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase-8; Caspase-8 subunit p10; Caspase-8 subunit p18; caspase8; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MGC78473; MORT1-associated ced-3 homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18, 41, 43 Source/Isotype: Rabbit IgG