CST, 98156SF, MAVS (E8Z7M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MAVS mouse. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, MAVS (E8Z7M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #98156) is ready to ship. Product Usage Information This product is the carrier free version of product #83000. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 83000 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MAVS (E8Z7M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MAVS protein. Non-specific nuclear staining in mouse brain and apical membrane staining of mouse stomach epithelium were observed by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala449 of mouse MAVS protein. Background The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF, and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6). Alternate Names CARD adapter inducing interferon beta; CARD adapter inducing interferon-beta; Cardif; D430028G21Rik; IFN-beta promoter stimulator-1; Interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS-1; Ips1; Mavs; MGC25836; mitochondrial anti-viral signaling protein; mitochondrial antiviral signaling protein; Mitochondrial antiviral-signaling protein; OTTMUSP00000017159; OTTMUSP00000017160; virus-induced signaling adapter; Virus-induced-signaling adapter; Visa Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG