CST, 98261W2, HPK1 (E1C3L) Rabbit Monoclonal Antibody (SignalFlex™ Pacific Blue™ Conjugate)

Monoclonal Antibody for studying HPK1. Highly specific and rigorously validated in-house, HPK1 (E1C3L) Rabbit Monoclonal Antibody (SignalFlex^™ Pacific Blue^™ Conjugate) (CST #98261) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity HPK1 (E1C3L) Rabbit mAb (SignalFlex™ Pacific Blue™ Conjugate) recognizes endogenous levels of total HPK1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HPK1 protein. Background Hematopoietic progenitor kinase 1 (HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the biological function of HPK1 (3,6,7). Alternate Names Hematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK1; M4K1; MAP4K1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen-activated protein kinase kinase kinase kinase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG