CST, 98271S, DELE1 (E9K6O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DELE1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DELE1 (E9K6O) Rabbit Monoclonal Antibody (CST #98271) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DELE1 (E9K6O) Rabbit Monoclonal Antibody recognizes overexpressed levels of total DELE1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DELE1 protein. Background DAP3-binding cell death enhancer 1 (DELE1) is a protein kinase activator that responds to mitochondrial stress as part of the integrated stress response (ISR) (1-4). The OMA1-DELE1-HRI ISR pathway is activated by cellular stressors such as iron deficiency, causing OMA1 to cleave DELE1 into a short form (DELE1(S) or S-DELE1), which accumulates in the cytosol and activates the protein kinase activity of EIF2AK1/HRI (1-4). Under stable cell conditions, DELE1 is quickly degraded (4). Alternate Names DAP3 binding cell death enhancer 1; DAP3-binding cell death enhancer 1; DAP3-binding cell death enhancer 1 short form; DAP3-binding cell death enhancer 1, long form; Death ligand signal enhancer; DELE; Dele1; DELE1(L); DELE1(S); hypothetical protein LOC9812; K0141; KIAA0141; S-DELE1; Uncharacterized protein KIAA0141, mitochondrial Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 60 Source/Isotype: Rabbit IgG