CST, 98354S, NMNAT1 (D7O4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NMNAT1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NMNAT1 (D7O4N) Rabbit Monoclonal Antibody (CST #98354) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NMNAT1 (D7O4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total NMNAT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala268 of human NMNAT1 protein. Background Nicotinamide mononucleotide adenylyl transferases (NMNATs) catalyze the reversible reaction of ATP with NaMN (nicotinic acid mononucleotide) or NMN (nicotinamide mononucleotide) to produce NaAD (nicotinic acid adenine dinucleotide) or NAD (nicotinamide adenine dinucleotide). NAD is an essential cofactor or substrates for many enzymes like PARP1 and Sirt1 that regulate diverse cellular processes including oxidative reactions and transcription. NMNATs maintain NAD levels for internal homeostasis (1,2). NMNAT1 is localized to the nucleus and loss-of-function mutant in mice causes embryonic lethality (3). In humans, several different NMNAT1 mutations are associated with Leber congenital amaurosis (LCA), the most common cause of inherited childhood blindness (4-7). Alternate Names LCA9; NaMN adenylyltransferase 1; Nicotinamide mononucleotide adenylyltransferase 1; nicotinamide nucleotide adenylyltransferase 1; Nicotinamide-nucleotide adenylyltransferase 1; Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1; Nicotinate-nucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NMN/NaMN adenylyltransferase 1; NMNA1; NMNAT; NMNAT1; PNAT1; pyridine nucleotide adenylyltransferase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG