CST, 9837S, ID3 (D16D10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Id3. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, ID3 (D16D10) Rabbit Monoclonal Antibody (CST #9837) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ID3 (D16D10) Rabbit Monoclonal Antibody recognizes endogenous levels of total ID3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ID3 protein. Background Inhibitor of DNA-binding/Differentiation (ID) proteins are a family of proteins that function to repress the activity of basic helix-loop-helix (bHLH) transcription factors. There are four known ID proteins in humans (ID1-4), all of which contain a helix-loop-helix domain but lack a basic DNA binding domain. Heterodimerization with bHLH transcription factors therefore functions to sequester bHLH proteins and prevent their binding to DNA (1). ID proteins play important functional roles in development, primarily by inhibiting premature differentiation of stem/progenitor cells (1,2). ID3 plays an important role in immune system development where it has been shown to repress E2A-mediated differentiation of T cells (3). Studies in mouse models have shown that homozygous deletion of ID3 disrupts regulatory T cell differentiation (4) and leads to development of γδ T cell lymphoma (5). Outside of the hematopoietic compartment, ID3 was shown to repress MyoD, implicating ID3 in TGFβ-mediated muscle repair (6). Similarly, research studies have shown that ID3 suppresses p21 in colon cancer cells, a function that is purported to promote the self-renewal capacity of putative cancer-initiating cells (7). Alternate Names 1R21; BHLHB25; Class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR-1; HEIR1; Helix-loop-helix protein HEIR-1; ID-like protein inhibitor HLH 1R21; ID3; Inhibitor of differentiation 3; Inhibitor of DNA binding 3; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein; inhibitor of DNA binding 3, HLH protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG