CST, 98423T, MAD2L2 (F9H9U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAD2L2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MAD2L2 (F9H9U) Rabbit Monoclonal Antibody (CST #98423) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MAD2L2 (F9H9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAD2L2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val203 of human MAD2L2 protein. Background Mitotic arrest deficient 2 like 2 (MAD2L2), also known as REV7, is a Mad family mitotic spindle assembly checkpoint protein that regulates progression into anaphase of mitosis (1), and functions in translesion DNA synthesis and DNA double-strand break (DSB) repair (2). MAD2L2 expression is high in human cancer, and expression levels are correlated with prognosis. MAD2L2 expression may predict the effectiveness of chemotherapy and PARP inhibitors, and MAD2L2 inhibitors may enhance the effects of these treatments (3). Alternate Names FANCV; hREV7; MAD2 (mitotic arrest deficient, yeast, homolog)-like 2; MAD2 mitotic arrest deficient-like 2; MAD2 mitotic arrest deficient-like 2 (yeast); MAD2-like protein 2; MAD2B; MAD2L2; MD2L2; mitotic arrest deficient 2 like 2; Mitotic arrest deficient 2-like protein 2; mitotic arrest deficient homolog-like 2; Mitotic spindle assembly checkpoint protein MAD2B; polymerase (DNA-directed), zeta 2, accessory subunit; POLZ2; REV7; REV7 homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit IgG