CST, 98492SF, FcR gamma (E6Y1A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FcER1G. Validated for WB,IHC,IHC,IF,IF. Highly specific and rigorously validated in-house, FcR gamma (E6Y1A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #98492) is ready to ship. Product Usage Information This product is the carrier free version of product #78401. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 78401 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity FcR gamma (E6Y1A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FcRγ protein. Non-specific staining was observed in normal human kidney and salivary gland by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FcRγ protein. Background FcRγ (Fc receptor gamma-chain, FcεRI gamma, High affinity immunoglobulin epsilon receptor subunit gamma) is a component of the IgE Fc receptor FcεRI, a tetramer of an alpha chain, a beta chain, and two disulfide-linked gamma chains (FcRγ) (1,2). FcεRI is expressed exclusively on basophils and mast cells and is involved in triggering allergic reactions (1-3). FcRγ also forms signaling complexes with other Fc receptors, including FcγRIA and FcγRIIIA. FcRγ shows high homology to the T cell receptor (TCR) ζ chain (4-6) and contains an immunoreceptor tyrosine-based activation motif (ITAM) which elicits a pro-inflammatory response (1-3). Alternate Names Fc epsilon receptor Ig; Fc fragment of IgE receptor Ig; Fc fragment of IgE, high affinity I, receptor for; Fc receptor gamma-chain; Fc-epsilon RI-gamma; FCER1G; FCERG; FceRI gamma; FCRG; FcRgamma; gamma polypeptide; High affinity immunoglobulin epsilon receptor subunit gamma; IgE Fc receptor subunit gamma; immunoglobulin E receptor, high affinity, gamma chain Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 12 Source/Isotype: Rabbit IgG