CST, 98543S, Phospho-Stat3 (Ser754) Antibody

Polyclonal Antibody for studying STAT3 (Ser754) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-Stat3 (Ser754) Antibody (CST #98543) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Stat3 (Ser754) Antibody recognizes endogenous levels of Stat3 protein only when phosphorylated at Ser754. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser754 of human Stat3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). Stat3 is phosphorylated by TBK1 at Ser754 in response to cytosolic DNA reducing Stat3 transcriptional activity (11). Alternate Names Acute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 86 SOURCE: Rabbit