CST, 98559T, NUP153 (E3I6Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NUP153. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, NUP153 (E3I6Z) Rabbit Monoclonal Antibody (CST #98559) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity NUP153 (E3I6Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUP153 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro428 of human NUP153 protein. Background The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1,2). Nucleoporin 153 kDa (NUP153) protein functions as a scaffolding element that recruits other proteins to form the "nuclear basket" on the nuclear side of the pore complex. NUP153 is essential for normal nucleocytoplasmic transport of proteins and mRNAs and is critical for the quality control and retention of unspliced mRNAs in the nucleus (1,2). NUP153 is a potential DNA-binding subunit of the NPC and is important for proper regulation of embryonic stem cell pluripotency and differentiation (1,3). Depletion of NUP153 leads to derepression of developmental genes and induction of early differentiation. NUP153 binds to Nucleoporin-Associated Regions (NARs) that are found near the transcriptional start sites of developmental genes and mediates recruitment of polycomb repressor complex 1 (PRC1) to repress transcription in embryonic stem cells (3). Alternate Names 153 kDa nucleoporin; HNUP153; N153; NU153; nuclear pore complex protein hnup153; Nuclear pore complex protein Nup153; nucleoporin 153; nucleoporin 153kD; nucleoporin 153kDa; Nucleoporin Nup153; NUP153 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 180 Source/Isotype: Rabbit IgG