CST, 98587T, MED1 (F6U2C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MED1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MED1 (F6U2C) Rabbit Monoclonal Antibody (CST #98587) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MED1 (F6U2C) Rabbit Monoclonal Antibody recognizes endogenous levels of total MED1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro999 of human MED1 protein. Background The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops' that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter and works to sustain proper chromatin architecture during active transcription (6-8). Alternate Names Activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; MGC71488; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARBP; PPARG binding protein; PPARGBP; RB18A; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting protein 2; Thyroid receptor-interacting protein 2; TR-interacting protein 2; TRAP220; TRIP-2; TRIP2; vitamin D receptor-interacting protein 230 kD; Vitamin D receptor-interacting protein complex component DRIP205 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit IgG