CST, 98649SF, Insulin Receptor beta (E9L5V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying InsR-beta. Validated for WB,IHC,IHC,IF,IF,F. Highly specific and rigorously validated in-house, Insulin Receptor beta (E9L5V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #98649) is ready to ship. Product Usage Information This product is the carrier free version of product #23413. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 23413 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Insulin Receptor beta (E9L5V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total insulin receptor β. The 50 kDa band(s) seen on western blot is a probable partial degradation product of insulin receptor β. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1374 of human insulin receptor. Background Insulin receptor (InsR) is a heterodimeric membrane receptor tyrosine kinase. It is composed of an extracellular α-subunit containing the ligand binding domain, a β-subunit containing an extracellular domain, a transmembrane domain, and a cytoplasmic tyrosine kinase domain (1). Binding of insulin to InsR results in receptor autophosphorylation and subsequent tyrosine kinase activation (2). This provides a docking site for various adaptor molecules, including insulin receptor substrate (IRS), Gab, and Shc, phosphorylation of which promotes subsequent activation of multiple downstream signaling pathways, including MAPK, PI3K, and TC10 (3,4). These events lead to increased glucose uptake and metabolism, and can promote cell growth. Loss-of-function mutation or desensitization of the InsR are two major contributors to insulin resistance and Type 2 diabetes (5). Alternate Names CD220; HHF5; INSR; Insulin receptor; Insulin receptor subunit alpha; Insulin receptor subunit beta; IR Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 95, 220 Source/Isotype: Rabbit IgG