CST, 98694SF, Dab2 (D7O9T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying DAB2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Dab2 (D7O9T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #98694) is ready to ship. Product Usage Information This product is the carrier free version of product #12906. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12906 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Dab2 (D7O9T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Dab2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly388 of human Dab2 protein. Background Disabled homolog 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is downregulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18). Alternate Names Adaptor molecule disabled-2; DAB adaptor protein 2; Dab, mitogen-responsive phosphoprotein, homolog 2; DAB2; DAB2, clathrin adaptor protein; Differentially expressed in ovarian carcinoma 2; Differentially-expressed protein 2; Disabled homolog 2; disabled homolog 2, mitogen-responsive phosphoprotein; disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila); DOC-2; DOC2; FLJ26626; mitogen-responsive phosphoprotein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 96 Source/Isotype: Rabbit IgG