CST, 98757T, Eosinophil peroxidase (F3C2H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EPX. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Eosinophil peroxidase (F3C2H) Rabbit Monoclonal Antibody (CST #98757) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Eosinophil peroxidase (F3C2H) Rabbit Monoclonal Antibody recognizes endogenous levels of total Eosinphil peroxidase protein. This antibody recognizes the full-length precursor, heavy chain, and light chain subunits of human Eosinophil peroxidase protein. Non-specific nuclear staining was observed in human testis and limited human tumors by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn585 of human Eosinphil peroxidase protein. Background Eosinophil peroxidase (EPX), also known as EPO, is a cationic protein stored in the secondary granulocytes of eosinophils (1-4). Unprocessed precursor EPX is an 80 kDa monomeric glycoprotein, which consists of two polypeptides linked by disulfide bonds: ~55 kDa heavy and ~15 kDa light chain (1-3, 6). In response to allergens or parasitic infection, eosinophils are recruited to affected tissues, where EPX catalyzes the formation of reactive oxidants lethal to tissue-invading parasites (1-4). In the tumor microenvironment of some breast cancers, eosinophils accumulate near blood vessels and infiltrate hypoxic regions, where EPX is released in high quantities by infiltrating immune cells (5). EPX may contribute to cancer progression by augmenting pro-tumorigenic collagen production and angiogenesis, making it a potential therapeutic target in breast cancer therapy (5). Alternate Names Eosinophil peroxidase; Eosinophil peroxidase heavy chain; Eosinophil peroxidase light chain; EPER; EPO; EPP; EPX; EPX-PEN; EPXD; PERE Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 81, 45-50, 20-25 Source/Isotype: Rabbit IgG