CST, 98778T, TMEM119 (E4B9S) Mouse Monoclonal Antibody

Monoclonal Antibody for studying TMEM119 mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM119 (E4B9S) Mouse Monoclonal Antibody (CST #98778) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity TMEM119 (E4B9S) Mouse Monoclonal Antibody recognizes endogenous levels of total TMEM119 protein by IF-Frozen but is not sensitive enough to detect endogenous levels by IF-IC. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse TMEM119 protein. Background Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (1). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (2). and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM). DAM are conserved in human and mouse and have been identified in Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and 5XFAD and APP/PS1 mouse models (3,4). This protein's specificity as a microglia marker has proven itself important for labeling microglia in healthy tissue as well as deciphering them from infiltrating macrophages and other cell types in neurodegenerative disease models (1,2). Alternate Names AW208946; BC025600; MGC38046; obif; Osteoblast induction factor; TM119; Tmem119; Transmembrane protein 119 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1 kappa