CST, 98783T, BAF (E4O8R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BAF. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, BAF (E4O8R) Rabbit Monoclonal Antibody (CST #98783) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 25880 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity BAF (E4O8R) Rabbit Monoclonal Antibody recognizes endogenous levels of total BAF protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala12 of human BAF protein. Background Barrier-to-autointegration factor (BAF or BANF1) plays a major role in the lifecycle of retroviruses, where it inhibits the reverse transcribed viral genome from integrating into itself (1-3). BAF exists as a dimer in solution, and serves as a bridge for double-stranded DNA in an ordered nucleoprotein complex (4). BAF interacts with LEM-domains of lamin binding proteins emerin and LAP2, and serves to help reform the nuclear envelope during telophase (5-7). The binding of BAF to its various partners is inhibited by Ser4 phosphorylation, which is deposited by vaccinia-related kinases (VRKs) and occurs during interphase and mitosis (8,9). Certain viral genomes encode kinases extremely similar to VRK to prevent BAF from binding to their DNA, impeding viral replication (10,11). BAF has been implicated in various tumor types and is associated with poor prognosis (12-14). Alternate Names BAF; BAF nuclear assembly factor 1; BANF1; barrier to autointegration factor 1; Barrier-to-autointegration factor; Barrier-to-autointegration factor, N-terminally processed; BCRG1; BCRP1; Breakpoint cluster region protein 1; D14S1460; MGC111161; NGPS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 10,11 Source/Isotype: Rabbit IgG