CST, 98923S, Sleeping Beauty Transposase Antibody

Polyclonal Antibody for studying SB10. Validated for Western Blotting. Highly specific and rigorously validated in-house, Sleeping Beauty Transposase Antibody (CST #98923) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Sleeping Beauty Transposase Antibody recognizes transfected levels of total Sleeping Beauty transposase protein. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu258 of Sleeping Beauty transposase protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Sleeping Beauty Transposase is part of a transposon system designed to allow viral free genetic insertion into vertebrate DNA. The system is composed of two components: a transposable element (transposon) that can carry DNA of interest, and a transposase that cuts and pastes the transposon into the genome. The transposase was identified from a consensus sequence of inactive Tc1/mariner-like transposase DNA sequences from salmonid fish. It was constructed by fusing and modifying two sequences from Atlantic salmon (Salmo salar) and one sequence from rainbow trout (Oncorhynchus mykiss). The transposon,T, was identified from a consensus sequence of extinct Tc-1 like transposons in salmonid fish (Tanichthys albonubes) (1). Further modifications of the system have been made since its initial construction: sequence changes to the transposase to fit better to an improved consensus sequence has increased its efficiency by 100 fold (2). Alternate Names SB transposase; SB10; Sleeping Beauty 10; Sleeping Beauty transposase; synthetic transposase Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 40 SOURCE: Rabbit