CST, 98941T, CD8 alpha (D4W2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD8A mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 3 sizes. Highly specific and rigorously validated in-house, CD8 alpha (D4W2Z) Rabbit Monoclonal Antibody (CST #98941) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 60168 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD8 alpha (D4W2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD8α protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp42 of mouse CD8α protein. Background Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). Alternate Names BB154331; CD8 antigen, alpha chain; Cd8a; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 35-42 Source/Isotype: Rabbit IgG