CST, 98946T, Granzyme K (E6Z6O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Granzyme K. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Granzyme K (E6Z6O) Rabbit Monoclonal Antibody (CST #98946) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Granzyme K (E6Z6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total Granzyme K protein. This antibody does not cross-react with human Granzyme A, B, H, or M proteins. This antibody cross-reacts with a 300 kDa protein of unknown identity in some cell extracts. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human Granzyme K protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Granzyme K has tryptase-like activity with a preference for cleaving its specific substrates after basic residues, arginine or lysine (4-7). Granzyme K is expressed by NK T cells, cytotoxic T cells, and NK cells (8,9). After perforin-mediated entry into a target cell, Granzyme K induces apoptosis via generation of reactive oxygen species, DNA damage, and BID-dependent mitochondrial damage (10-12). Alternate Names Fragmentin-3; GRAK; granzyme 3; Granzyme K; granzyme K (granzyme 3; granzyme K (serine protease, granzyme 3; Granzyme-3; GZMK; NK-Tryp-2; NK-tryptase-2; TRYP2; tryptase II; tryptase II) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 17-27 Source/Isotype: Rabbit IgG