CST, 99023SF, ATPIF1 (D6P1Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ATPIF1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, ATPIF1 (D6P1Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #99023) is ready to ship. Product Usage Information This product is the carrier free version of product #13268. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13268 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ATPIF1 (D6P1Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ATPIF1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala46 of human ATPIF1 protein. Background The ATPase inhibitor factor 1 (ATPIF1) gene encodes a mitochondrial ATPase inhibitor that limits ATP depletion when mitochondrial respiration is impaired (1). ATPIF1 becomes activated following a drop in pH, binding to β-F1-ATPase, thereby inhibiting the hydrolase activity of the H -ATP synthase (1,2). In addition to its role as an ATP hydrolase, ATPIF1 has also been shown to play a regulatory role in cellular energy metabolism by triggering the induction of aerobic glycolysis in cancer cells resulting in their Warburg phenotype (3,4). Research studies demonstrate that the overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis (3). Alternate Names ATIF1; ATP synthase F1 subunit epsilon; ATP synthase inhibitor protein; ATP synthase inhibitory factor subunit 1; ATP5IF1; ATPase inhibitor protein; ATPase inhibitor, mitochondrial; ATPase inhibitory factor 1; ATPI; ATPIF1; ATPIP; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase; IP; MGC1167; MGC8898 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 12 Source/Isotype: Rabbit IgG