CST, 99086S, CDC40 (E4V4J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDC40. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, CDC40 (E4V4J) Rabbit Monoclonal Antibody (CST #99086) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CDC40 (E4V4J) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDC40 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro459 of human CDC40 protein. Background CDC40/ PRP17 (Pre-mRNA-processing factor 17) is associated with the activated spliceosome, and is required for pre-mRNA splicing. CDC40 is necessary for efficient G1/S and G2/M transitions, linking splicing to control of cell cycle progression (1,2).CDC40 expression is induced in hepatitis B virus (HBV)-infected hepatocellular carcinoma (HCC) cells through NF-κB and miR-1269b, promoting malignancy in HCC (3). Alternate Names CDC40; cell division cycle 40; Cell division cycle 40 homolog; cell division cycle 40 homolog (S. cerevisiae); EH-binding protein 3; EHB3; FLJ10564; hPRP17; MGC102802; pre-mRNA splicing factor 17; Pre-mRNA-processing factor 17; PRP17; PRP17 homolog; PRPF17 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG