CST, 99105T, TDG (E5T5G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TDG. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TDG (E5T5G) Rabbit Monoclonal Antibody (CST #99105) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TDG (E5T5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total and SUMOylated TDG protein. This antibody may detect a band of unknown origin at 200 kDa in some rodent and monkey cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TDG protein. Background Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established by two enzymes, DNMT3A and DNMT3B, and is maintained by DNMT1 (3,4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7). Knockout or catalytic inactivation of TDG leads to embryonic lethality, due in part to the loss of DNA methylation patterns at the promoters and enhancers of developmental genes (8,9). TDG is commonly SUMOylated at Lys330, although the exact consequence of this modification is not yet fully understood. SUMOylation has been reported to help TDG dissociate from its abasic product, thereby increasing catalytic turnover (10-12). Additional studies suggest that SUMOylation affects TDG's cellular localization or lowers its base excision activity, allowing it to act as a ‘reader' protein for 5-fC and 5-caC modified DNA (13-14). Alternate Names G/T mismatch-specific thymine DNA glycosylase; hTDG; TDG; thymine DNA glycosylase; Thymine-DNA glycosylase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 58, 60 Source/Isotype: Rabbit IgG