CST, 99136S, NFS1 (F1E7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NFS1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NFS1 (F1E7S) Rabbit Monoclonal Antibody (CST #99136) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NFS1 (F1E7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total NFS1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NFS1 protein. Background Cysteine desulfurase (NFS1) is a component of the core complex of the iron-sulfur cluster (ISC) assembly machinery in the mitochondrial matrix (1). This enzyme desulfurates L-cysteine. The sulfur from cysteine is used for the biosynthesis of ISCs (2,3). Studies show that high levels of NFS1 are essential to cancer cells when oxygen levels are above the 3-8% oxygen concentration typical of most tissues. Strong NFS1 expression is necessary for primary lung tumor growth and NFS1 protects lung adenocarcinomas from ferroptosis in reaction to oxidative damage (2). In addition, suppression of NFS1 expression leads to growth inhibition of some breast cancer cells (3). Alternate Names Cysteine desulfurase; Cysteine desulfurase, mitochondrial; HUSSY-08; IscS; NFS1; NFS1 cysteine desulfurase; NFS1 nitrogen fixation 1 homolog; NFS1 nitrogen fixation 1 homolog (S. cerevisiae); NIFS; nitrogen fixation 1 (S. cerevisiae, homolog); nitrogen-fixing bacteria S-like protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG