CST, 99188T, TLE3 (E4F3Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TLE3. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TLE3 (E4F3Y) Rabbit Monoclonal Antibody (CST #99188) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity TLE3 (E4F3Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total TLE3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro427 of human TLE3 protein. Background Transducin-like enhancer of split proteins (TLE1, TLE2, TLE3, TLE4, and TLE6) are mammalian homologs of Drosophila Groucho (1). TLEs contain several WD-repeats implicated in protein-protein interaction (2,3). TLEs are transcriptional co-repressors that bind to many transcription factors, such as LEF1, Runx1, Oct-1, hepatocyte nuclear factor 3-β as well as histone H3 (4-7). TLEs are differentially expressed during animal development and may have overlapping as well as distinct functions (8). Alternate Names enhancer of split groucho 3; Enhancer of split groucho-like protein 3; ESG; ESG3; FLJ39460; GRG3; HsT18976; KIAA1547; TLE family member 3, transcriptional corepressor; TLE3; transducin like enhancer of split 3; transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 3, homolog of Drosophila E(sp1); Transducin-like enhancer protein 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG