CST, 99235T, NDUFB9 (E7W6K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NDUFB9. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NDUFB9 (E7W6K) Rabbit Monoclonal Antibody (CST #99235) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NDUFB9 (E7W6K) Rabbit Monoclonal Antibody recognizes endogenous levels of total NDUFB9 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NDUFB9 protein. Background NDUFB9 is an accessory subunit in complex I (NADH: ubiquinone oxidoreductase) of the respiratory chain in mitochondria (1). It is shown that a mutation in the gene leads to reduced NDUFB9 protein levels and lower complex I activity, indicating a role of this mutation in mitochondrial complex I deficiency (2). In addition, research studies using affinity purification and mass spectrometry show that ORF9c of SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2) interacts with NDUFB9 in HEK-293T/17 cells when ORF9c is expressed in HEK-293T/17 cells (3). Alternate Names B22; CI-B22; complex I B22 subunit; Complex I-B22; DKFZp566O173; FLJ22885; LYR motif-containing protein 3; LYRM3; MC1DN24; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH-ubiquinone oxidoreductase B22 subunit; NADH:ubiquinone oxidoreductase subunit B9; NDUB9; NDUFB9; UQOR22 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG