CST, 99293S, Myoferlin (E9Z4X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying myoferlin. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Myoferlin (E9Z4X) Rabbit Monoclonal Antibody (CST #99293) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Myoferlin (E9Z4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total myoferlin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human myoferlin protein. Background Myoferlin (MYOF) is a type II transmembrane protein of the ferlin family found mainly in muscle and endothelial cells (1,2). The protein has multiple C2 domains located at its N-terminal cytoplasmic region, which mediate lipid binding and protein-protein interactions (3). Myoferlin is localized to various membrane structures, including the plasma membrane and vesicle membranes, to promote vesicle trafficking (4,5), membrane fusion and repair (6), angiogenesis (7), and adipogenesis (8). High expression of myoferlin has been found in various cancer types and is associated with poor prognosis (3,9). Myoferlin contributes to cancer progression by promoting angiogenesis, vasculogenic mimicry, energy metabolism reprogramming, epithelial-mesenchymal transition, and exosome formation. Inhibition of myoferlin has been proposed as a potential therapeutic treatment for cancer (10-12). Alternate Names fer-1-like 3, myoferlin; fer-1-like family member 3; Fer-1-like protein 3; FER1L3; FLJ36571; FLJ90777; KIAA1207; MYOF; Myoferlin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 250 Source/Isotype: Rabbit IgG