CST, 99376S, Cystatin F/CST7 (F9R1W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CST7 mouse. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Cystatin F/CST7 (F9R1W) Rabbit Monoclonal Antibody (CST #99376) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cystatin F/CST7 (F9R1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total cystatin F/CST7 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant mouse cystatin F/CST7 protein. Background Cystatin F, also known as CST7, is an endosomal-lysosomal cysteine protease inhibitor member of the cystatin type 2 superfamily, encoded by the gene (1). It is selectively expressed by hemopoietic cells such as cytotoxic T cells, natural killer cells, monocytes, and dendritic cells, suggesting a role in immune regulation (1,2). CST7 is expressed as an inactive dimer and converted to a functionally active monomer localized to the endo-lysosomal compartment (3). CST7 has been implicated in various cancer types, including breast and cervical cancer, and is thought to play a role in modulating the tumor microenvironment (4,5). Additionally, CST7 is highly upregulated in disease-associated microglia in Alzheimer's disease and other neurodegenerative diseases, and may play a sex-specific role in neurodegeneration (6,7). Alternate Names CMAP; Cst7; cystatin 7; cystatin F (leukocystatin); Cystatin-7; Cystatin-F; cystatin-like metastasis-associated protein; CYTF; leukocystatin; MGC151424; MGC151426 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 15-25 Source/Isotype: Rabbit IgG