CST, 99481SF, HS1 (E6G7M) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying HS1 mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, HS1 (E6G7M) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #99481) is ready to ship. Product Usage Information This product is the carrier free version of product #16621. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 16621 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity HS1 (E6G7M) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total HS1 protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels. This antibody also recognizes a non-specific band of unknown origin at 25 kDa in mouse tissue extracts. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu310 of mouse HS1 protein. Background HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7). Alternate Names AW213261; Hcls1; hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; Hs1; LckBP1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Mouse IgG1 kappa