CST, 9953S, Staurosporine

Chemical Modulators for studying in the research area. Product Usage Information Dissolve the lyophilized alkaloid in DMSO or Methanol. For a 1 mM solution, dissolve in 0.536 mL. Storage Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Background Staurosporine is an alkaloid isolated from the culture broth of Streptomyces staurosporesa. It is a potent, cell permeable protein kinase C inhibitor with an IC50 of 0.7 nM. At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK) (1). At 50-100 nM, it is a functional neurotrophin agonist, promoting neurite outgrowth in neuroblastoma, pheochromocytoma and brain primary neuronal cultures. At 0.2- 1 μM, staurosporine induces cell apoptosis (2,3). Alternate Names alkaloid; apoptosis; apoptosis induction; CAMKII; cleaved caspase; cleaved parp; Cleaved-Caspase3; cleaved-PARP; full length PARP; leaved caspase3; mlc; MLCK; myosin light chain; neurotrophin agonist; nhibitor; PKA; pkc; PKC; PKC inhibitor; PKG; staurosporene; Streptomyces staurosporesa