CST, 99633S, HOIP/RNF31 (E6M5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HOIP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HOIP/RNF31 (E6M5B) Rabbit Monoclonal Antibody (CST #99633) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HOIP/RNF31 (E6M5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOIP/RNF31 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HOIP/RNF31 protein. Background HOIL-1-interacting protein (HOIP/RNF31), a RING-type E3 ubiquitin ligase, is the catalytic subunit of the Linear Ubiquitin Chain Assembly Complex (LUBAC) that is associated with TNF-R1 (1). Research studies have shown that the LUBAC consists of three subunits: HOIP, HOIL-1L, and Sharpin that facilitate canonical NF-kB activation in response to pro inflammatory cytokines through M1-linked linear ubiquitination of NEMO and RIP1 (2-6). As part of the LUBAC, HOIP has also been implicated in the negative regulation of interferon-mediated antiviral signaling through the suppression of RIG-I activation (7). The role of HOIP in LUBAC function and human disease is underscored by naturally occurring mutations in HOIP that impair LUBAC assembly and NF-kb activation. Patients that are homozygous for this mutation in HOIP have multi organ auto inflammation and immunodeficiency (8). Alternate Names E3 ubiquitin-protein ligase RNF31; FLJ10111; FLJ23501; HOIL-1-interacting protein; HOIP; MGC19975; Paul; RING finger protein 31; RING-type E3 ubiquitin transferase RNF31; RNF31; ZIBRA; Zinc in-between-RING-finger ubiquitin-associated domain protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG