CST, 99680T, LOXL2 (E3P7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LOXL2. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, LOXL2 (E3P7Y) Rabbit Monoclonal Antibody (CST #99680) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 72903 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity LOXL2 (E3P7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total LOXL2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LOXL2 protein. Background Lysyl oxidase-like 2 (LOXL2) is a secreted copper-dependent amine oxidase and a member of the lysyl oxidase family (1). It primarily catalyzes the oxidation of lysine (or hydroxylysine) residues in collagen and elastin to form peptidyl aldehyde derivatives (1). These modifications are required for further cross-linking of target proteins to enhance extracellular matrix (ECM) stiffness. LOXL2 has been associated with progression and metastases of many cancer types, including breast cancer and fibrotic disease, all through active remodeling of the ECM (2-5). Additionally, within the cell, LOXL2 has been shown to play a role in EMT, cell polarity, and transcriptional repression (6-8). LOXL2 has become an attractive target for therapeutic intervention in cancer treating, blocking metastasis, and halting fibrosis (9-10). Alternate Names LOR; LOR2; LOXL2; Lysyl oxidase homolog 2; lysyl oxidase like 2; lysyl oxidase related 2; lysyl oxidase-like 2; lysyl oxidase-like 2 delta e13; lysyl oxidase-like 2 protein; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14; WS9-14 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG