CST, 99756T, HPSE (E3N3H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HPSE. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HPSE (E3N3H) Rabbit Monoclonal Antibody (CST #99756) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HPSE (E3N3H) Rabbit Monoclonal Antibody recognizes endogenous levels of total HPSE protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu466 of human HPSE protein. Background Heparanase (HPSE) is an endo-β-D-endoglycosidase that catalyzes the cleavage heparan sulfate (HS) side chains from proteoglycans at the cell surface (1). The protein is synthesized as a 65 kDa inactive precursor that undergoes proteolytic processing, yielding 8 kDa and 50 kDa protein subunits that heterodimerize to form an active enzyme (2). HPSE serves as a key modulator of extracellular matrix (ECM) basic structure. The cleavage of HS from proteoglycans and associated changes at the ECM also cause the release of growth factors from the matrix to bind to their cognate receptors, leading to activation of downstream signaling pathways (3). A small percentage of HPSE localizes to specialized lipid raft regions. There, HPSE activates multiple signaling pathways, such as Akt, ERK, and Src pathways, by a nonenzymatic mechanism mediated by its C-terminal domain (4,5). HPSE is involved in the development of diseases such as fibrosis initiation, inflammation, tumor progression, and viral infection (6-9). HPSE inhibitors have been developed and pursued as novel therapeutics for these diseases (10-12). Alternate Names Endo-glucoronidase; HEP; Heparanase; Heparanase 50 kDa subunit; Heparanase 8 kDa subunit; heparanase exon 9&10 deletion; heparanase exon10-deletion; Heparanase-1; HPA; HPA1; HPR1; HPSE; HPSE1; HSE1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50, 65 Source/Isotype: Rabbit IgG