CST, 99797S, Influenza A Nucleoprotein (NP) (F8L6X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Nucleoprotein (strain A/Russia:St.Petersburg/8/2006 H1N1). Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Influenza A Nucleoprotein (NP) (F8L6X) Rabbit Monoclonal Antibody (CST #99797) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Influenza A Nucleoprotein (NP) (F8L6X) Rabbit Monoclonal Antibody recognizes endogenous levels of total influenza A virus (Russia:St.Petersburg/8/2006 H1N1) nucleoprotein. This antibody cross-reacts with nucleoprotein from H1N1, H3N2, and H5N1, and will broadly react with influenza A subtypes. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala85 of influenza A virus (Russia:St.Petersburg/8/2006 H1N1) nucleoprotein (Uniprot #B4URE0). Background Influenza, or flu, is a contagious respiratory virus of the Orthomyxoviridae family, which is subdivided into four genera: A, B, C, and D (1). Influenza A is the most common flu virus causing infections in humans and is the source of seasonal epidemics and occasional pandemics (2). The main proteins encased in the viral nucleocapsid are hemagglutinin (H) and neuraminidase (N), from which viral subtype names are derived (3,4). Influenza A subtypes H1N1 and H3N2 are the source of seasonal infections in humans and are regularly vaccinated against (2). The influenza A nucleoprotein (NP) plays a key role in the viral replication and lifecycle. NP forms ribonucleoprotein complexes that are essential for the transcription and replication of the virus. As the NP is highly conserved across influenza A subtypes, it has become a target for both vaccine development and antiviral drug development (5,6). Host protein ZBP1 directly binds to viral NP, inducing necroptosis of infected cells via the RIPK3 pathway (7). NP can inhibit the innate immune response by binding to mitochondrial antiviral signaling protein (MAVS) and Toll-interacting protein (Tollip), activating mitophagy and subsequent MAVS degradation (8). Alternate Names nucleocapsid protein; nucleoprotein; protein N Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG