CST, 99825SF, CXCR5 (D6L3C) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CXCR5. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CXCR5 (D6L3C) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #99825) is ready to ship. Product Usage Information This product is the carrier-free version of product #72172. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 72172 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CXCR5 (D6L3C) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CXCR5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of human CXCR5 protein. Background CXCR5 is a G protein-coupled receptor belonging to the chemokine receptor subfamily (1). Upon binding of its ligand, the chemokine CXCL13, CXCR5 initiates multiple intracellular signaling pathways that regulate cell proliferation, survival, and migration (2). CXCR5 is expressed in both mature B cells and follicular helper T cells, and responds to CXCL13 gradient to control lymphocyte migration toward secondary lymphoid tissues (3). CXCR5 has also been shown to be highly expressed in primary breast tumors, in correlation with their propensity to grow and metastasize (4). Alternate Names BLR1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; C-X-C chemokine receptor type 5; C-X-C motif chemokine receptor 5; CD185; chemokine (C-X-C motif) receptor 5; CXC-R5; CXCR-5; CXCR5; MDR-15; MDR15; MGC117347; Monocyte-derived receptor 15 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG